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Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants
Published on: October 15, 2019
RNA Chemical Modifications in Mammalian Skeletal Muscle Development, Homeostasis, and Disease: Regulatory Mechanisms
Dujun Chen1,2,3, Mailin Gan1,2,3, Yuhang Lei1,2,3
1Farm Animal Germplasm Resources and Biotech Breeding Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China.
Abstract:
Skeletal muscle is a core component of the motor system, responsible for movement, posture maintenance, and energy metabolism. It plays a vital role in physiological stress, injury repair, and metabolic homeostasis. In recent years, with the advancement of epigenetic research, RNA modification has become a hot topic in skeletal muscle biology. This article systematically reviews the progress in the study of RNA modification in regulating the fate of skeletal muscle, focusing on the dynamic regulatory mechanisms and pathophysiological significance of N6-methyladenosine (m6A) modification in muscle differentiation, regeneration, and atrophy. It also briefly introduces the existing evidence for 5-methylcytidine (m5C), adenosine-to-inosine (A-to-I) editing, and pseudouridine (Ψ) in skeletal muscle. A comprehensive understanding of these mechanisms is crucial for elucidating the physiological regulation of skeletal muscle and the pathogenesis of related diseases and also provides a new direction for the development of intervention strategies.
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