Related Experiment Video
Updated: Aug 28, 2026

Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
Published on: August 21, 2017
BTV Remodels the oISG15-Association Proteome
Di Kang1, Qinxue Gao1, Mingxin Zhang1
1College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
Abstract:
ISG15 is an interferon-induced ubiquitin-like protein that exerts diverse functions during viral infection. We previously reported that ovine ISG15 (oISG15) promoted bluetongue virus (BTV) replication by stabilizing viral NS1 and VP4 in an ISGylation-independent manner, although the mechanism remained unknown. Here, using the generated anti-oISG15 antibody, we performed immunoprecipitation (IP) coupled with label-free Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) to characterize the oISG15-associated proteome in BTV-infected and uninfected cells. The results showed that BTV infection markedly remodeled the oISG15 association landscape, with decreased enrichment of several autophagy- and trafficking-related proteins and increased enrichment of mitochondrial metabolic proteins in oISG15 immunoprecipitates. Functionally, oISG15 overexpression induced modest alterations in autophagy-related markers in uninfected cells yet attenuated these markers' abundance during BTV infection. Together, this study revealed BTV-induced changes in the oISG15-associated proteome that coincided with alterations in autophagy-related markers.
More Related Videos
Related Concept Videos
Proteomics
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Protein Complexes with Interchangeable Parts
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complexes with Interchangeable Parts
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The Proteasome Structure
The proteasome is an...
Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...

