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Updated: Aug 29, 2026

Quantitative Characterization of Liquid Photosensitive Bioink Properties for Continuous Digital Light Processing Based Printing
Published on: April 14, 2023
Proposition for the analysis of background toner distribution to sequence ink lines and printing with a statistical
Mathieu Arès1, R Brent Ostrum2, Zain Bhaloo2
1Département de Biochimie, Chimie, Physique et Science Forensique, Université du Québec à Trois-Rivières, Trois-Rivières, Québec, Canada.
Abstract:
Determining the sequence of handwriting and laser-printed text on documents that do not intersect is a recurring challenge in forensic document examination. This study proposes using background toner particle distribution analysis combined with kernel density estimation and likelihood ratio calculation to evaluate printing-writing sequence. Toner particle density was measured by manual counting under near-infrared microscopic imaging at 200× magnification across controlled conditions using three black-toner laser printers. Toner particle density within pen strokes differed significantly by sequence, while intra-page location, distance from pen strokes, and writing orientation showed no significant effect. Pages containing printed text exhibited significantly higher background toner density in unprinted regions than blank pages. A diminishing depletion effect was observed across successive paper layers in stacked printing. The proposed framework provides a quantitative and transparent approach to sequence determination, with a Student's t-test alternative when reference materials are unavailable.

