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Published on: May 1, 2014
Two near-complete assemblies reveal R-subgenome structural and centromeric divergence associated with reproductive
Yang Liu1, Wanna He1,2, Chunhui Wang1
1State Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China.
Abstract:
We present two near-complete triticale genome assemblies and perform pan-centromere analyses across >200 haplotypes from parental species, synthetic allopolyploids, cultivars, and 337 resequenced accessions, revealing a shared chromatin framework underlying functional centromeres in all three subgenomes, where wheat- and rye-derived CENH3 co-occupy divergent centromeric repeats. Postpolyploid evolution drives extensive centromere remodeling, including size convergence, repeat turnover, and locus repositioning, with the R subgenome emerging as the primary source of structural and centromeric divergence. In F1 hybrids, R-subgenome structural and centromeric divergence is associated with meiotic abnormalities, including chromosome misalignment, lagging chromosomes, and micronuclei formation, suggesting a link between centromere variation and reduced meiotic stability.
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