Protein Analyte Sensing with an Outer Membrane Protein G (OmpG) Nanopore

Nanopore sensing is a powerful lab-on-a-chip technique that allows for the analysis of biomarkers present in small sample sizes. In general, nanopore clogging and low detection accuracy arise when the sample becomes more complex such as in blood or lysate. To address this, we developed an OmpG nanopore that distinguishes not only different proteins in a mixture but also protein homologues. Here, we describe this OmpG-based nanopore system that specifically analyzes target biomarkers in complex mixtures.