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Updated: Aug 30, 2026

Monitoring Pedogenic Inorganic Carbon Accumulation Due to Weathering of Amended Silicate Minerals in Agricultural Soils.
Published on: June 4, 2021
Elevated CO2 increases C allocation to CaCO3 mineralization relative to net organic C fixation in Ceratostigma
Long Guo1, Cailei Liu1, Ting Lei1
1College of Landscape Architecture, Sichuan Agricultural University, Chengdu, 611130, China.
Abstract:
Ceratostigma willmottianum, which can mineralize atmospheric CO2 directly into CaCO3 for long-lasting C sequestration, has both organic and inorganic C sequestration functions. However, how elevated CO2 affects CaCO3 mineralization and the allocation of C between the two C sequestration remains unclear. Therefore, C. willmottianum was exposed to five CO2 concentrations (400, 550, 700, 850, and 1000 ppm) in combination with 13C isotope labeling, carbonic anhydrase (CA) inhibitor treatments, and transient overexpression assays to systematically investigate these effects. Results showed that 700 ppm CO2 was the most effective in promoting plant growth, photosynthesis, and CaCO3 accumulation, whereas 1000 ppm CO2 exerted an inhibitory effect. 13C tracing revealed that elevated CO2 increased C allocation to CaCO3 mineralization from 3.03% (400 ppm) to 4.62% (700 ppm), while decreasing that to net organic C fixation from 63.40 to 60.51%. CA inhibitor experiments further indicated that extracellular CA plays a key role in promoting more C allocation to CaCO3 mineralization. Gene expression and functional validation identified CwβCA2 as a key gene responding to elevated CO2 and promoting CaCO3 mineralization. This study provides a theoretical basis and candidate gene resources for developing biomineralization-based C capture and storage technologies to cope with rising CO2 in the future.
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