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Updated: Aug 31, 2026

Development of Multiplex Real-Time RT-qPCR Assays for the Detection of SARS-CoV-2, Influenza A/B, and MERS-CoV
Published on: November 10, 2023
Viral respiratory infections and their co-occurrence among patients hospitalized in Ayatollah Rouhani Hospital,
Alireza Firouzjahi1, Samaneh Rouhi1, Mohammad Ranaee1
1Clinical Research Development Unit of Rouhani Hospital, Babol University of Medical Sciences, Babol, Iran.
Background:
Vaccination has reduced the viral respiratory diseases in the community, but it has not ended. This study was conducted to determine the distribution of COVID-19, the influenza virus, and the human respiratory syncytial virus (RSV).
Methods:
This retrospective, descriptive-analytical study was conducted based on patient file information in Babol City (2021-2024). Clinical examination of the patient, computed tomography (CT), and real-time polymerase chain reaction (PCR) were used to identify the viruses in 10,567 patients. Mean, standard deviation (SD), distribution (%), and logistic regression were used to analyze the data using SPSS-23 (p<0.05).
Results:
83.34% and 16.65% of patients suffered from COVID-19 (12.95% ≥ 50 years old) and influenza (15.42%, ≤ 50 years old), respectively. The most underlying diseases were related to cardiovascular disease (17.47%). Female patients suspected of COVID-19 (females; 14.14% versus (vs) males; 11.87%) and influenza (females; 14.90% vs males; 15.62%) were more than male patients. COVID-19 (12.91%) and influenza (15.27%) in 2022 and 2023 showed a high distribution, respectively. Co-occurrence of COVID-19 and influenza was observed in one female. Gender, season, and year (each with a P-value of P=0.00) was associated in this descriptive analysis with the distribution of COVID-19. A significant relationship between season and year with the distribution of influenza was observed.
Conclusion:
A high distribution of COVID-19 was diagnosed among patients. Gender and year of study had a significant effect on COVID-19. Diagnosis and differentiation of the viral agent by molecular methods should be performed in a patient.
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