3D finite element analysis of the effect of cytoskeleton crosslinking on osteocyte mechanical transmission based on
Zhuang Han1,2, Shou-Hui Wang3, Bo Huo2
1Beijing Kupei Sports Culture Corp Ltd, Beijing, China.
Abstract:
The cytoskeleton mediates osteocyte responses to fluid shear stress from matrix deformation. Under simulated microgravity, cytoskeleton depolymerizes, while Spectrin-MS provides compensatory structural support. We modeled osteocytes with zero to three crosslinking levels under 1.5 Pa shear. Increased crosslinking raised membrane displacement (+2.60%) and stress (+20.71%), but reduced nuclear displacement (-12.93%) and stress (-10.86%). Cytoskeleton displacement and stress decreased with more crosslinking, whereas Spectrin-MS compensated. Crosslinking alters the intracellular mechanical environment, with Spectrin-MS playing a compensatory role, enhancing understanding of osteocyte mechanosensitivity. .
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