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Mixture Analysis at Micromolar Concentrations with 2D Benchtop NMR Enhanced by SABRE Hyperpolarization
Bono O Jimmink1, Mattia Negroni1, Thom B Posthumus1
1Institute for Molecules and Materials (IMM), Radboud University, Heyendaalseweg 135, Nijmegen6525 AJ, The Netherlands.
Abstract:
Because of its low cost and portability, benchtop NMR has become increasingly popular over the last years. However, these low-field instruments lack sensitivity and spectral resolution, which limits their application in real-world problems, particularly at low concentrations. Here, we combine SABRE hyperpolarization with two-dimensional NMR spectroscopy on a benchtop spectrometer to gain sensitivity and spectral resolution. We employ a novel cosubstrate approach, utilizing an α-amino acid, which facilitates measurements at low micromolar concentrations. This approach enabled the complete resolution of a mixture of six N-heteroaromatic compounds at concentrations of 5 μM each, within 20 min. The power of this methodology was further tested on an extract of ground coffee, for which several analytes - estimated in the low micromolar concentration range - were identified.
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