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Orthogonal Protein Purification Facilitated by a Small Bispecific Affinity Tag
Published on: January 16, 2012
Protein tags facilitate the development of bifunctional molecules
Shimei Chen1, Yuting Xin1, Qidong You1
1State Key Laboratory of Natural Medicines and Jiangsu Key Laboratory of Drug Design and Optimization, China Pharmaceutical University, Nanjing 210009, China; Department of Medicinal Chemistry, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
Abstract:
Bifunctional molecules are a leading trend in drug development today, but the lack of small-molecule ligands greatly limits their development. Within this landscape, protein tags have played a pivotal role in advancing the field, enabling work from initial proof-of-concept validation to in-depth mechanistic research and further exploration of biological effects. In this review, we systematically summarize and analyze the various protein tags employed in the development of bifunctional molecules, aiming to help researchers in the field select suitable protein tags for their studies. Furthermore, we offer a perspective on the future application of protein tags in bifunctional molecule development.
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