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Updated: Sep 2, 2026

In Planta Gene Expression and Gene Editing in Moso Bamboo Leaves
Published on: August 18, 2023
The DlamiR156-DlaSPL60-DlaP5CS1 cascade directly regulates proline synthesis, conferring drought tolerance in Ma
Nannan Wang1, Yang Cheng2, Zihan Yan1
1Basic Forestry and Proteomics Center (BFPC), College of Forestry, HaiXia Institute for Science and Technology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, China.
Abstract:
Drought stress disrupts cellular homeostasis and severely limits plant growth. Although proline accumulation is a major adaptive response to water deficit, how drought-responsive regulatory networks control proline biosynthesis in woody grasses remains poorly understood. Here, we combined genetic manipulation, molecular assays, and physiological analyses to investigate the function of the DlamiR156-DlaSPL60 module in Ma bamboo (Dendrocalamus latiflorus Munro). We show that DlamiR156 acts as a negative regulator of drought tolerance by repressing DlaSPL60 via transcript cleavage, whereas drought-induced reduction of DlamiR156 releases this repression; DlaSPL60 directly binds to the DlaP5CS1 promoter and activates its transcription, thereby promoting proline accumulation. Our findings establish a mechanistic link between the conserved miR156-squamosa promoter-binding protein-like regulatory module and proline biosynthesis in bamboo, providing new insights into how post-transcriptional regulation coordinates metabolic reprogramming during drought adaptation in perennial woody grasses.
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