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Ultrafast Dynamics of rsCherryRev in Comparison with mCherry Reflecting Its Reversive Switchability
Yu-Sheng Tsai1, Sheng-Ting Hung2, Chuang-Yu Lin3
1Department of Electrophysics, National Yang Ming Chiao Tung University, Hsinchu300, Taiwan.
Abstract:
Ultrafast dynamics of a red fluorescence protein mCherry and its reversibly switchable mutant rsCherryRev was studied by using a 10 fs visible pulse laser and fastscan transient absorption spectroscopy system. This mutation of mCherry for rsCherryRev has caused ultrafast relaxation with lifetimes of ∼0.3 ps and ∼3 ps much faster than the lifetime of mCherry (∼0.5 ns). The ultrashort lifetimes of rsCherryRev are considered to reflect the initial structural change of the chromophore during photoisomerization. The ultrashort duration of 10 fs laser pulse also visualizes that the C═C stretching mode intensity increases at ∼0.7 ps delay, reflecting the timing of photoisomerization in rsCherryRev.
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