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Metabolomics Data Analysis with the HMDB
Yamilé López-Hernández1,2, Prashanthi Kovur1, David S Wishart3,4,5,6
1Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada.
Abstract:
The Human Metabolome Database (HMDB) is a web-based resource that supports metabolite identification and interpretation for human studies. This chapter outlines five protocols: (1) identifying metabolites using mass spectrometry with retention time or collision cross-section data (MS-RT/CCS); (2) identifying metabolites using tandem mass spectrometry (MS/MS); (3) identifying metabolites using nuclear magnetic resonance (NMR); (4) comparing measured concentrations to HMDB reference values; and (5) interpreting metabolomics results via HMDB's MetaboCards and PathBank links.
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