Related Experiment Video
Updated: Sep 3, 2026
07:09
A Rapid and Efficient Method for Assessing Pathogenicity of Ustilago maydis on Maize and Teosinte Lines
Published on: January 3, 2014
Chlorazol Black E Staining of Plant Tissue Infected with Ustilago maydis to Monitor Pathogenic Development On and In
Nora Marie Kühne1, Kai Heimel2
1Georg-August-University Goettingen, Institute of Microbiology and Genetics, Department of Microbial Cell Biology Grisebachstr, Goettingen, Germany.
Methods in Molecular Biology (Clifton, N.J.)
|September 2, 2026
Abstract:
The routine analysis of mutant strains of Ustilago maydis includes monitoring of fungal pathogenic growth. Defined developmental stages including filamentous growth on the leaf surface, appressoria formation, and plant penetration, as well as hyphal proliferation in planta can be visualized using a straight forward and low-cost staining procedure using Chlorazol Black E staining.
