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Updated: Sep 3, 2026

Inoculating and Observing Arbuscular Mycorrhizal Cultures on Superabsorbent Polymer-Based Autotrophic Systems
Published on: June 14, 2024
Asymbiotic Growth of Arbuscular Mycorrhizal Fungi
Kayo Hashimoto1, Sachiko Tanaka1, Masayoshi Kawaguchi2,3
1Division of Symbiotic Systems, National Institute for Basic Biology, Okazaki, Aichi, Japan.
Abstract:
The development of asymbiotic culture methods for arbuscular mycorrhizal (AM) fungi is crucial for studying their symbiotic mechanisms and developing new inoculants. AM fungi lack genes encoding cytosolic fatty acid synthases, and thus require plant-derived lipids for their propagation. Certain fatty acids, such as myristate, can promote their mycelial growth and sporulation without a host plant. In this chapter, we describe detailed methods for asymbiotic culture using fatty acids and strigolactone.
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