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Updated: Sep 3, 2026

Generation of Recombinant Human IgG Monoclonal Antibodies from Immortalized Sorted B Cells
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Cloning of Antibodies from Patients with Heparin-Induced Thrombocytopenia

Lu Zhou1,2, Nathan D Witman1,2, Wen Zhu1,2,3

  • 1Versiti Blood Research Institute, Milwaukee, WI, USA.

Methods in Molecular Biology (Clifton, N.J.)
|September 2, 2026
PubMed

We discuss two antibody cloning protocols-single B-cell culture (Nojima culture) and single B-cell expression cloning-to isolate antibodies associated with heparin-induced thrombocytopenia (HIT) from patients displaying clinical manifestations of the condition. The objective of the antibody cloning study is to provide valuable insights into the molecular mechanisms underlying the B-cell response and antibody-mediated pathogenesis in HIT, aiming to improve the diagnosis and management of this condition.

Keywords:
AntibodyB-cellsCloningHeparin-induced thrombocytopeniaNojima cultureRecombinantSingle B-cell expression cloning

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