Image Analysis Approaches for In Situ Hybridization Technologies

In situ hybridisation (ISH) is an invaluable experimental technique when considering the holistic process of transcription through to translation. Without good tools to identify sequence-based structures within cells and tissues, researchers are left with only half of the story when looking at protein expression. While there are many experimental approaches, including a rainbow of chromogens and a choice of brightfield and fluorescent approaches, the Image Analysis of ISH-based experiments remains a significant block to many looking to explore these datasets. This chapter aims to highlight some of the considerations when approaching the image analysis of ISH experiments, as well as showing a step-by-step walkthrough for analysis using tools developed by Visiopharm, an industry leader in digital pathology image analysis with over 20 years of experience in the field.