Convergent Synthesis of a Bifunctional GPI as a Universal Platform to Access Diverse GPI and GPI-Anchored Protein
Xin Yan1, Rajendra Rohokale1, Zhongwu Guo1,2
1Department of Chemistry, University of Florida, Gainesville, Florida32611, United States.
Abstract:
A bifunctional glycosylphosphatidylinositol (GPI) anchor containing an azide and a biotin to facilitate additional functionalization via click chemistry and avidin conjugation, respectively, was synthesized by a convergent strategy using an orthogonal protection tactic. It is a general platform to rapidly access diverse GPI and GPI-anchored protein (GPI-AP) probes. Spin-labeled GPI and GPI-AP derived from this platform were used in a related study to show that GPI governs the membrane orientation of GPI-AP, validating GPI as a unique protein anchor.
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