[Development of an Analytical Method for Simultaneous Determination of Deoxynivalenol and Ochratoxin A in Wheat]

A simultaneous analytical method was developed for the determination of deoxynivalenol (DON) and ochratoxin A (OTA) in wheat. Analytes were purified from wheat extracts by a multifunctional column and quantified by LC-MS/MS. An interlaboratory study was conducted to validate the analytical method using three spiked and two contaminated wheat samples. The ranges of mean recoveries of DON and OTA were 88-89% and 91-96%, respectively. The RSDs for repeatability and reproducibility were all ≤15%. Furthermore, the levels of DON and OTA in artificially contaminated wheat samples were analyzed by the simultaneous analytical method. The results showed that the developed analytical method yielded analytical values equivalent to those obtained when analyzing DON and OTA individually by existing analytical methods. Based on these results, the simultaneous analytical method for DON and OTA in wheat developed in this study is considered suitable as an alternative to the methods for analyzing each mycotoxin individually.