Related Experiment Video
Updated: Sep 4, 2026
Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
Published on: December 7, 2012
Adaptation of the Yield-Scaling Factor to Reduce Target Yield Deviation and Donation Time in Pathogen-Reduced
Marco Amato1, Anita Siller1, Lisa Seekircher2
1Central Institute for Blood Transfusion and Immunology, University Hospital of Innsbruck, Tirol Kliniken GmbH, Innsbruck, Austria.
Background:
Platelet concentrates from apheresis donations are indispensable in transfusion medicine. Integration of pathogen reduction (PR) technologies into platelet manufacturing requires adjustment of apheresis devices to ensure consistent platelet yields while minimizing donation time. We investigated the suitability of Sysmex XN1000 (Sysmex Corporation, Kobe, Japan) in blood bank mode (BBM) for measuring platelet concentrates to optimize the yield-scaling factor (YSF) of apheresis devices, thereby standardizing target yields and reducing donation time.
Methods:
We compared measurements with the Sysmex XN530 and XN1000 BBM and evaluated three Trima Accel (Terumo, Tokyo, Japan) YSF setups (YSF 0.90/XN530, YSF 0.90/XN1000, and YSF 1.05/XN1000). Differences in yield deviation and donation time were assessed using linear mixed-effects models adjusted for target yield with robust SEs.
Results:
We analyzed 4,383 platelet apheresis procedures from 1,573 donors across setups in large-volume (LV) and dual-storage (DS) collections. Compared with YSF 0.90/XN530, YSF 0.90/XN1000 and YSF 1.05/XN1000 significantly reduced yield deviation by 0.26 and 0.41×1011 platelets/unit (95% confidence interval [CI] 0.18-0.34 and 0.35-0.48, respectively) in LV collections and by 0.34 and 0.24×1011 platelets/unit (95% CI 0.24-0.45 and 0.01-0.47, respectively) in DS collections. Compared with YSF 0.90/XN530, YSF 1.05/XN1000 reduced the donation time by 8.09 min (6.93-9.26) and 9.88 min (6.81-12.94) for LV and DS collections, respectively.
Conclusions:
YSF 0.90/XN1000 and YSF 1.05/XN1000 reduced yield deviation and donation time than did YSF 0.90/XN530, supporting the use of XN1000 BBM to calibrate Trima Accel apheresis devices and can help in standardizing target yields while reducing donation time in PR.
