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Updated: Sep 4, 2026
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Identification of Protein Interacting Partners Using Tandem Affinity Purification
Published on: February 25, 2012
Identification of eIF4ET binding partners in C. elegans
Judith Tafur1, Angelica Morales Cueva1, Nicole E Familiari1
1Cell Biology, The University of Texas Southwestern Medical Center, Dallas, TX, United States.
Micropublication Biology
|September 3, 2026
Abstract:
The eIF4ET family protein IFET-1 is needed for oocyte development and long-term storage in C. elegans . How IFET-1 plays multiple roles in oocyte biology is unclear. Using pull-downs and mass spectrometry, we identified proteins that interact with IFET-1 . We show that three IFET-1-interacting partners- PENR-1 , SQD-1 , and LSM-4 -are essential for producing viable oocytes in C. elegans females.
