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Updated: Sep 4, 2026
MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
Published on: October 7, 2025
Tetrahedral DNA-guided catalytic hairpin assembly for reference-calibrated intracellular microRNA imaging and sensing
Jiaqi Li1,2, Yi Sun1,2, Lijuan Qi1,3
1Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Jilin, P.R. China. qilijuan@ciac.ac.cn.
Abstract:
We report a tetrahedral DNA platform that positions a catalytic hairpin assembly within an optimal spatial confinement window, thereby maximizing target-triggered amplification relative to background leakage. AS1411-mediated targeting and a β-actin reference channel further enable selective, internally calibrated imaging and sensing of intracellular microRNA-21.
