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Modulatory effects of methyl gallate on the inflammatory response in gout arthritis via P2X7 receptor
Felipe M S Pereira1,2,3, Luana B Correa1,3, Leonardo N Seito1
1Fundação Oswaldo Cruz, Laboratory of Applied Pharmacology, Farmanguinhos, Rua Sizenando Nabuco, 100, Manguinhos, 21041-250 Rio de Janeiro, RJ, Brazil.
Abstract:
Gout is an inflammatory disease caused by monosodium urate (MSU) crystal deposition, triggering NLRP3 inflammasome activation, interleukin-1β (IL-1β) release, and P2X7 receptor-mediated amplification of inflammation. This study evaluated the anti-inflammatory effects of methyl gallate (MG) and its interaction with the P2X7 receptor. MG reduced IL-1β production in activated macrophages, inhibited P2X7-dependent membrane pore formation, and produced effects similar to those of the selective P2X7 antagonist A740003. In a murine gout model, MG decreased joint edema, leukocyte accumulation, and IL-1β levels. Computational analyses demonstrated stable MG binding to P2X7, highlighting its potential as a therapeutic candidate for gout treatment.
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