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Single-Molecule Measurement of Protein Interaction Dynamics Within Biomolecular Condensates
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Observation of hidden dynamics underlying facilitated dissociation of proteins from DNA

Ridvan Nepravishta1, Junji Iwahara1

  • 1Department of Biochemistry & Molecular Biology, Sealy Center for Structural Biology & Molecular Biophysics, University of Texas Medical Branch, Galveston, Texas 77555-1068, USA. j.iwahara@utmb.edu.

Chemical Communications (Cambridge, England)
|September 4, 2026
PubMed

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...