A Technique for the Generation of Mucosal and Acral Melanoma Cell Lines

Mucosal and acral melanomas are rare subtypes of melanoma with unique clinical and biological features. A dearth of well-established and characterized melanoma cell lines that reflect rare subtypes impedes research on these lethal diseases. We present a protocol developed by exploring various combinations of established techniques that we used to generate mucosal and acral cell lines from patient tissues and patient derived xenografts.