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Updated: Sep 7, 2026

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RNA Isolation of Pseudomonas aeruginosa Colonizing the Murine Gastrointestinal Tract
Published on: September 28, 2011
A rapid two-step protocol for isolation of DNA-free, high-integrity RNA from gram-negative Bacteria
Ravi Chauhan1, Varsha Kaushik1, Aakruti Patel1
1Microbiology Lab, School of Life Sciences, Central University of Gujarat, Kundhela, Vadodara, Gujarat, India.
Journal of Microbiological Methods
|September 5, 2026
Abstract:
A two-step protocol employing Tris-equilibrated phenol (pH 4.5) and sodium dodecyl sulfate was optimized to efficiently isolate DNA-free, intact RNA from gram-negative bacteria. This method reduced processing time and reagent use, thereby minimizing RNA degradation and contamination. The extracted RNA was suitable for gene expression profiling.
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