Spectrum meets sustainability: green-validated novel simultaneous assays of antimicrobial fixed dose combination

Sustainability, affordability and instant reproducible responses are urgently targeted for quality control of drug analyses. Synergistic antimicrobial combination of a cephalosporin and an antiprotozoal in a single pill, is much convenient and manoeuvrable. Earlier chromatographic attempts of mixture analysis are reported. Herein, the first green spectrophotometric assay of pharmaceutical formulation of two antibiotics is done simultaneously; Cefuroxime Axetil and Ornidazole. The fixed dose combination is determined without prior separation. Q-Absorbance Isosbestic point methodology, coupled with first derivative photometric analysis, are applied for drugs' assay in synthetic binary mixtures of different concentration ratios. Validation in regard to international council for harmonization is confirmed prior to methods' application to laboratory prepared tablets. Limits of quantitation reached down to 1.509 µg mL⁻¹ (CFUR-X at the isosbestic point), 2.094 µg mL⁻¹ (ORNI at the isosbestic point), and 2.364 µg mL⁻¹ (ORNI by first derivative). The proposed techniques, when compared statistically with the reported HPLC method, declared insignificant differences. As an environmental obligation, the proposed method's greenness was compared to the reported HPLC and HPTLC procedures using Analytical Eco-Scale and AGREE metrics. The first spectrophotometric simultaneous assay of CFUR-X and ORNI via isosbestic as well as ¹D shows higher greenness scores preceding the previous chromatographic ones, which assures ecofriendly, simple and applicable analysis.