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Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
Published on: August 3, 2018
LC-MS/MS-Based Lipidomic Profiling Reveals the Regulatory Effect of Stemoninoamide on TGF-β1-Induced Myofibroblast
Wen Kang1,2, Xueting Peng1,3, Wenbin Zhou1,2,4
1Key Laboratory of Mass Spectrometry Imaging and Metabolomics (Minzu University of China), State Ethnic Affairs Commission, Beijing, China.
Abstract:
The dried roots of Stemona tuberosa, known as "Bai-Bu" in traditional Chinese medicine, have been employed as a therapeutic agent for the treatment of pertussis and tuberculosis. The chemical composition of S. tuberosa is dominated by alkaloids which have been reported to possess antifibrosis effect. Herein, ELISA kit and immunofluorescence analyses showed that the alkaloid of stemoninoamide (Stem) isolated from S. tuberosa exhibited inhibitory effect on the production of α-SMA and Col-1 of HFL1 cells stimulated by TGF-β1. Further lipidomic analysis based on LC-MS/MS revealed differential metabolites via multivariate statistical analysis. Pathway enrichment analyses indicated that differential metabolites were mostly implicated in S1P/S1PR signaling pathway, which could be potentially associated with the antifibrosis mechanism of Stem.
