γ-Linolenic Acid Inhibits Contractile Responses in Pig Basilar Arteries via Prostanoid TP Receptor Antagonism and...

γ-Linolenic acid (GLA) is an n-6 polyunsaturated fatty acid (PUFA) with reported vasoprotective effects. GLA has been shown to inhibit contractions in pig coronary arteries via competitive antagonism at prostanoid TP receptors. α-Linolenic acid (ALA), an n-3 PUFA and a structural isomer of GLA, has been shown to inhibit contractions in pig basilar arteries (PBAs) via prostanoid TP receptor antagonism and activation of K⁺ channels. However, the actions of GLA in cerebral arteries remain unclear. In this study, we examined the effects of GLA on contractile responses in PBAs. GLA (10^-5-10^-4 M) concentration-dependently inhibited contractions induced by the TP receptor agonist U46619 (3 × 10^-8 M) and prostaglandin F_2α (PGF_2α; 3 × 10^-6 M). GLA (3 × 10^-6-3 × 10^-5 M) shifted the concentration-response curve for U46619 to the right. Schild analysis yielded a slope of 1.33 (95% confidence interval: 0.70-1.96), which was not significantly different from unity, but GLA also reduced the maximal response to U46619. The apparent pA₂ value was 5.43. GLA (10^-4 M) also inhibited PGF_2α-induced contractions in the presence of the TP receptor antagonist SQ 29,548 (10^-6 M); this inhibitory effect was attenuated by Ba²⁺ (10^-3 M) but not by other tested K⁺ channel inhibitors. GLA also inhibited endothelin-1 (10^-8-3 × 10^-8 M)-induced contractions, and this effect was attenuated by Ba²⁺. These findings indicate that GLA, like ALA, suppresses contractile responses in PBAs through dual mechanisms involving TP receptor antagonism and activation of Ba²⁺-sensitive K⁺ channels.