[Rapid high performance liquid chromatography-tandem mass spectrometry method for the detection of 62 psychotropic...

Objective: To establish a rapid method for 62 psychotropic drugs in plasma using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) . Methods: In January 2025, plasma samples were extracted by methanol∶acetonitrile (1∶1, V/V), and then gradient eluted using a C18 column with an aqueous solution containing 5 mmol/L ammonium acetate and 5 mmol/L ammonium acetate in methanol as the mobile phase. Positive and negative ion rapid switching scanning modes were used, and segmented multi-reaction monitoring mode (MRM) was used for analysis. Quantification was performed by the external standard method. Results: There were a good linear relationships for 62 psychotropic drugs in plasma with correlation coefficients ranging from 0.9976 to 0.9999. The recovery rates of spiked samples at low, medium and high concentrations ranged from 90.7% to 107.9%, with relative standard deviations for intra-batch and inter-batch precision ranging from 0.9% to 11.1%. Conclusion: This method is simple, fast, and suitable for clinical poisoning detection, and for the simultaneous determination of 62 psychotropic drugs in plasma.