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Updated: Sep 10, 2026

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis
05:47

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis

Published on: April 4, 2025

Protocol for assessing endolysosomal chloride channel function in mammalian cells using endolysosomal patch-clamp

Yayu Wang1, Lily Yeh Jan1

  • 1Department of Physiology, University of California, San Francisco, San Francisco, CA 94158, USA.

STAR Protocols
|September 8, 2026
PubMed

Keywords:
BiophysicsCell BiologyMolecular Biology

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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...