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Accumulation of polyadenylated mRNA during liver regeneration

V Atryzek, N Fausto

    Biochemistry
    |April 3, 1979
    PubMed

    Insights

    During liver regeneration, cytoplasmic polyadenylated mRNA (poly(A)+-mRNA) significantly increased before DNA synthesis. This rise in mRNA occurred independently of changes in poly(A) tract length or recruitment of existing mRNA into polysomes.

    Area of Science:

    • Molecular Biology
    • Hepatology
    • Cellular Biology

    Background:

    • Liver regeneration involves complex gene expression regulation.
    • Polyadenylated mRNA (poly(A)+-mRNA) plays a crucial role in protein synthesis.
    • Understanding mRNA dynamics is key to comprehending liver repair.

    Purpose of the Study:

    • To investigate changes in polyadenylated mRNA during rat liver regeneration.
    • To determine if mRNA size or poly(A) tract length are altered.
    • To assess the recruitment of cytoplasmic mRNA into polysomes.

    Main Methods:

    • Partial hepatectomy in rats to induce liver regeneration.
    • Quantification of nuclear and cytoplasmic polyadenylated mRNA.
    • Analysis of mRNA size and poly(A) tract length.
    • Assessment of polysomal association of mRNA.

    Main Results:

    • Cytoplasmic poly(A)+-mRNA increased by 120% before DNA synthesis onset.
    • Nuclear polyadenylated RNA showed a modest increase (15-20%).
    • No significant changes in mRNA size or poly(A) tract length were observed.
    • Polysomal poly(A)+-mRNA increased faster than rRNA.

    Conclusions:

    • Liver regeneration involves a substantial increase in cytoplasmic poly(A)+-mRNA.
    • This increase precedes DNA synthesis and is not due to altered mRNA processing.
    • Existing cytoplasmic mRNA is not the primary source for the early increase in polysomal mRNA.

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