High-sensitive determination of midazolam in urine samples using hydrogen peroxide-free enhanced
Farzane Ahmadi1, Naader Alizadeh2
1Department of Chemistry, Faculty of Basic Sciences, Tarbiat Modares University, P.O. Box 14115-175, Tehran, Iran.
Abstract:
This paper proposes an enhanced electrochemiluminescence (ECL) nanostructure sensor for midazolam (Mdz) determination via one-step electropolymerization of the conductive molecularly imprinted polymer (CMIP) based on polypyrrole (PPy) on a carbon paste electrode (CPE) by the cyclic voltammetric (CV) technique. The FESEM technique showed that the surface of the sensor is made of nano-sized particles. In the absence of hydrogen peroxide, ECL signals of luminol were investigated and an enhanced response was observed by adding Mdz to the solution. Optimal experimental conditions were obtained for the proposed sensor. Its ECL behavior was investigated and the reaction mechanism was discussed. The calibration curve of the CMIP sensor was obtained in the dynamic linear range from 10 to 400 nM with a limit of detection (LOD) of 6 nM for the Mdz determination in solution. The good selectivity of the proposed CMIP sensor was shown by investigating the effect of some interferences on the CMIP and non-imprinted polymer electrode. This sensor was able to determine the amount of Mdz in urine samples with acceptable sensitivity.
