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Related Experiment Videos

Transepithelial permeability in the rabbit pancreas.

J W Jansen, J J de Pont, S L Bonting

    Biochimica Et Biophysica Acta
    |February 20, 1979
    PubMed
    Summary

    The rabbit pancreas

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    Area of Science:

    • Physiology
    • Gastroenterology
    • Cell Biology

    Background:

    • The transepithelial permeability of the pancreas is crucial for fluid and electrolyte balance.
    • Understanding the mechanisms governing pancreatic secretion is vital for treating related diseases.

    Purpose of the Study:

    • To investigate the permeability characteristics of the isolated rabbit pancreas.
    • To determine the effect of cholinergic stimulation on pancreatic permeability.

    Main Methods:

    • Utilized radioactive markers to assess the permeability of isolated rabbit pancreas to various molecules.
    • Measured solute concentrations in secreted fluid after incubation with and without carbachol.
    • Investigated the role of extracellular space and the effect of atropine.

    Main Results:

    • Pancreatic permeability to solutes is size-dependent, with smaller molecules like urea and glycerol passing readily.
    • Cholinergic stimulation with carbachol significantly increased the permeability to larger molecules (mannitol, lactose, sucrose, inulin).
    • Atropine blocked the carbachol-induced increase in permeability to sucrose, suggesting a cholinergic pathway.

    Conclusions:

    • The transcellular transport route in the rabbit pancreas is influenced by molecular size.
    • Cholinergic agents can modulate pancreatic permeability through a dose-dependent, likely extracellular, mechanism.
    • This permeability increase is independent of enzyme secretion itself.

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