Related Experiment Video
Updated: Sep 12, 2026

In vitro Digestion of Emulsions in a Single Droplet via Multi Subphase Exchange of Simulated Gastrointestinal Fluids
Published on: November 18, 2022
The Influence of Enzymatically Synthesized Medium- and Long-Chain Triacylglycerols on Lipid Digestion
Chenyu Jiang1,2, Yixiao Huang1,2, Xinyi Cheng1,2
1State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi214122, China.
Abstract:
Age-related declines in gastrointestinal function increase the malnutrition risk and highlight the need for lipid formulations with digestive and metabolic characteristics suited to older populations. In this study, a structurally characterized medium- and long-chain triacylglycerol (MLCT) prepared by enzymatic transesterification was compared with a compositionally equivalent physical blend (PB) using a simulated in vitro gastrointestinal digestion model for the elderly and an in vivo indirect calorimetry in adult mice. MLCT exhibited a more gradual fatty acid release pattern and 33-44% lower lipolysis during the initial gastrointestinal stages than PB without affecting final hydrolysis, and it significantly improved polyunsaturated fatty acid bioaccessibility. In mice, MLCT administration was associated with comparatively gradual changes in whole-body energy expenditure and respiratory gas exchange among the lipid treatments. These findings indicate that the TAG structure influences digestive kinetics and acute whole-body metabolic responses, supporting the further evaluation of MLCT for nutritional applications involving age-related digestive decline.
Related Concept Videos
Lipid Digestion
Lipid Absorption
These breakdown products bind with bile salts and lecithin to form micelles, which quickly pass between microvilli to come in close contact with the apical...
Overview of Lipid Metabolism
Lipolysis: The Breakdown of Lipids:
Lipolysis is the process of breaking down lipids, particularly triglycerides, into glycerol and fatty acids. This process typically occurs in the adipose tissue and is triggered by various hormones, including glucagon and...
Blood Studies for Cardiovascular System III: Serum Lipid Profile
Serum lipids are fats and fatty substances in the blood and are crucial for various bodily functions, including energy storage, cellular structure, and hormone production. Serum lipids consist of cholesterol, triglycerides, and phospholipids.
Cholesterol is a soft, fat-like substance found in all body cells. It is crucial for producing hormones, vitamin D, and substances that aid...
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...

