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Updated: Sep 12, 2026

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
Published on: February 9, 2012
Emerging trends of fluorescence lifetime imaging microscopy (FLIM): advances, challenges, and prospects
Baoyi Wang1, Meilin Li1, Xiaoshuai Huang2
1National Biomedical Imaging Center, College of Future Technology, Peking University, Beijing 100871, China.
Abstract:
This mini-review provides a concise overview of the latest advances in fluorescence lifetime imaging microscopy (FLIM). It discusses both time-domain and frequency-domain techniques, analysis methods - including phasor approaches and deep learning, and highlights applications in multiplexed imaging and quantitative biosensing. Furthermore, FLIM-empowered multimodal imaging approaches aimed at enhancing spatial and temporal resolution are discussed. Persistent challenges, including photon efficiency, probe sensitivity, and achieving high-speed imaging in live-cell environments, are critically assessed, outlining pathways toward future innovations.
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