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Application of a Novel Hyaluronan Hydrogel for Three-Dimensional Follicle Culture and Methodology for Mouse Ovarian Follicle Cryopreservation
Published on: May 9, 2025
Efficacy of Hyaluronan-Enriched Transfer Medium in Frozen-Thawed Embryo Transfer: A Retrospective Single-Center
Shoko Toyoda1, Seung Chik Jwa1, Suguru Kamishiro1
1Department of Obstetrics and Gynecology Jichi Medical University Tochigi Japan.
Objective:
Since 2023, our university hospital has incorporated hyaluronan-enriched transfer medium (HETM) into all frozen-thawed embryo transfers (FETs). This study aimed to evaluate whether HETM improved clinical pregnancy rates in FET, particularly in patients with recurrent implantation failure (RIF).
Methods:
We analyzed 368 FET cycles using a conventional hyaluronan-free transfer medium and 358 FET cycles using HETM, in which thawed embryos were incubated for 2-4 h before transfer. The main outcome was clinical pregnancy. Multiple logistic regression was used to evaluate the effectiveness of HETM.
Results:
The mean age of patients was 37.4 years (standard deviation = 4.2), and RIF, defined as failure to achieve pregnancy after two or more previous embryo transfers, accounted for 51.2%. Clinical pregnancy rates were significantly higher in the HETM group (37.7%) than in the control group (27.2%) (p = 0.002). Stratified analyses showed that HETM was associated with higher clinical pregnancy rates in RIF cycles (adjusted odds ratio = 2.4 [95% confidence interval: 1.4-3.9]). An additional analysis restricted to patients with three or more previous failed embryo transfers showed similar results.
Conclusion:
This study demonstrates HETM use is associated with improved clinical pregnancy rates in FET, particularly in cases of RIF.
