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Updated: Sep 15, 2026

Cryopreserving and Recovering of Human iPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
Published on: July 15, 2010
Clinical-Grade Cryopreservation of Human-Induced Pluripotent Stem Cells
Binnur Eroglu1, Stephanie Revoredo1, Ali Eroglu1,2
1Department of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA, USA.
Abstract:
Human-induced pluripotent stem cells (hiPSCs) can be derived from a variety of biopsy samples and have an unlimited capacity for self-renewal and differentiation into almost any cell type in the body. Therefore, hiPSCs offer unprecedented opportunities for patient-specific cell therapies, modeling of human diseases, biomarker discovery, and drug testing. To realize the full potential of clinical applications of hiPSCs, xeno-free and ideally chemically defined methods are required for their generation, expansion, and cryopreservation. In this chapter, we present a chemically defined and xeno-free slow freezing method for hiPSCs along with a chemically undefined protocol. Both approaches yield satisfactory post-thaw viability and cell growth.
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