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Updated: Sep 16, 2026
Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue
Published on: August 6, 2009
Preparation of Postmortem Human Formalin-Fixed Paraffin-Embedded Frontal Cortex Tissue for Profiling Pyramidal
Aleksandra Stanisavljevic1,2, Stephen D Ginsberg3,4,5,6
1Center for Dementia Research, Nathan Kline Institute, Orangeburg, NY, USA.
Abstract:
Digital spatial profiling (DSP) facilitates RNA sequencing (RNA-seq) of small populations of cells, specifically ~20 pyramidal neurons in postmortem formalin-fixed paraffin-embedded (FFPE) human brain tissue. This approach enables precise expression profiling via RNA-seq data linked to spatially characterized samples utilizing tissue-bound probes. We present a comprehensive protocol for manual slide preparation using the GeoMx DSP system as preliminary steps to spatial characterization of lamina-specific pyramidal neurons in human postmortem Brodmann area 9 (BA9) frontal cortex brain tissue.
