Quantification of Chikungunya Virus RNA by Real-Time RT-PCR Assay

RT-qPCR is characterized by‌ high sensitivity, strong specificity, excellent reproducibility, and operational simplicity, ‌enabling‌ rapid quantification of viral RNA within a short timeframe. The real-time PCR system relies upon the measurement of a fluorescent reporter during PCR, in which the amount of emitted fluorescence is directly proportional to the amount of the PCR product in a reaction. Here, we describe the use of TaqMan® probe-based one-tube Real-time reverse transcription quantitative polymerase chain reaction (RT-qPCR) for the detection and quantification of the Chikungunya virus (CHIKV) RNA.