Related Experiment Video
Updated: Sep 16, 2026

An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
Published on: October 4, 2018
Molecular Characterization of Infectious Laryngotracheitis Virus from Broiler Diseased Chickens in Northern Vietnam
Giang Thi Huong Tran1,2, Vuong Trong Tran3, Amonpun Rattanasrisomporn4
1Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi 131000, Vietnam.
Abstract:
PCR-based surveillance identified the infectious laryngotracheitis virus (ILTV) genome in 8 out of 14 (57.14%) commercial broiler farms across three Northern Vietnam provinces. Partial sequencing of the infected cell protein 4 (ICP4) and glycoprotein B, G, and J (gB, gG, and gJ) genes from the ILTV isolates obtained during 2023-2025 indicated that eight Vietnamese ILTV-positive samples were field ILTV strains. Additionally, high nucleotide identity was observed among the isolates, with ranges of 99.74-100% for the gB, 99.63-100% for the gJ, 99.66-100% for the gG, and 99.34-100% for the ICP4 genes. Phylogenetic analyses based on four genes (gB, gJ, gG, and ICP4) revealed that the eight ILTV strains belonged to two distinct lineages, except for the gJ gene. Analysis of the ICP4 gene showed that the five ILTV strains (VNUA-ILTV-02, VNUA-ILTV-05 to VNUA-ILTV-08) clustered with the United States Laryno Vac, Poulvac-ILT, and ON-2462242/1987 strains, whereas the remaining strains (VNUA-ILTV-01, VNUA-ILTV-03, and VNUA-ILTV-04) were in a distinct group. Furthermore, several amino acid changes were observed in the gB, gG, and gJ proteins of the characterized ILTV strains.

