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Evaluation of ESBL screening agar media for detecting ESBL-producing thymidine-dependent small-colony variants of
Tatsuya Negishi1, Takehisa Matsumoto2, Tomu Kamijo1
1Department of Laboratory Medicine, Shinshu University Hospital, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
Journal of Microbiological Methods
|September 15, 2026
Abstract:
We evaluated extended-spectrum β-lactamase (ESBL) screening agar media using 11 clinical Enterobacterales thymidine-dependent small-colony variants, including three ESBL-producing Escherichia coli. All three grew on Vital Media ESBL/MBL screening agar medium, two weakly on ChromID ESBL, and none on CHROMagar ESBL. Adding 10 μg/mL thymidine improved growth on the latter two.
