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Updated: Sep 17, 2026

Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
Optimizing yeast surface display efficiency: a multifaceted strategy analysis
Fanyu Chen1, Chaowei Zhou1, Peifang Ren1
1National Engineering Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, P.R. China.
Abstract:
Microbial cell surface display systems represent a promising and robust approach for engineering whole-cell biocatalysts, with broad applications in biotechnology and biomedicine. This methodology overcomes limitations in substrate transport, eliminates the laborious and time-consuming process of enzyme purification, and enhances reusability and regenerability in repeated batch processes, thereby delivering economic benefits. Among various host systems, yeast surface display (YSD) offers unique advantages, such as large cell size, ease of culture and genetic manipulation, and the capacity for complete post-translational processing of secreted proteins. This review highlights strategies to improve the efficiency of YSD from novel perspectives, including intracellular molecular transcription, cell surface engineering, and external spatial expansion. Additionally, it comprehensively summarizes the practical applications of YSD in biocatalysis, underscoring their transformative potential in industrial and biomedical contexts.
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