LIM domain-containing proteins Zyxin and LPP localize to apical epithelial cell-cell junctions at regions of stable
Katherine K Tjoelker1, Yashar Bashirzadeh2, Leah M Beel3
1Cellular & Molecular Biology Graduate Program, University of Michigan, Ann Arbor, MI.
Abstract:
LIM domain-containing proteins, Zyxin and Lipoma-Preferred Partner (LPP), localize to sites where actin filaments are strained, including actin stress fibers, focal adhesions, and cell-cell junctions. Utilizing the Xenopus laevis embryonic epithelium and live confocal microscopy, this study demonstrates that Zyxin and LPP localize to apical cell-cell junctions in polarized epithelia. Furthermore, particle image velocimetry (PIV) analysis reveals an inverse correlation between Zyxin/LPP intensity and F-actin velocity, suggesting that Zyxin and LPP are present at regions of stable F-actin.
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