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Selective curing of the endogenous plasmid pSF3 encoding organophosphorus hydrolase in Sphingobium fuliginis ATCC
Satoshi Ishikawa1, Natsumi Ishikawa1, Satoshi Onodera2
1Faculty of Pharmacy, Iryo Sosei University, Iwaki, Fukushima, Japan.
Bioscience, Biotechnology, and Biochemistry
|September 16, 2026
Abstract:
Sphingobium fuliginis ATCC 27551 degrades organophosphorus pollutants by organophosphorus hydrolase (OPH) encoded on a plasmid pSF3. The replacement plasmid with the same replication-associated genes was used to cure pSF3. By the selection of antibiotic resistance, pSF3 was eliminated without changing other plasmids. The cured strain would be useful to construct a strain capable of large-scale production of OPH.
