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CorrelationCalculator and Filigree: Tools for Data-Driven Network Analysis of Metabolomics Data
Published on: November 10, 2023
Network propagation in bipartite metabolite-reaction graphs for metabolomic data exploration
Julia Kuligowski1,2, Marta Moreno-Torres3,4,5, David Pérez-Guaita6
1Spanish Network in Maternal, Neonatal, Child and Developmental Health Research (RICORS- SAMID) (RD24/0013/0014), Neonatal Research Group, Health Research Institute La Fe, Valencia, Spain.
Introduction:
Interpretation of metabolomic data is frequently limited by incomplete metabolite coverage and the predefined representation of biochemical organization provided by pathway-based approaches.
Objectives:
This study describes and evaluates a graph-based network propagation method designed to faciitate metabolomic data exploration by diffusing node-level statistical relevance scores across a metabolic network.
Methods:
An undirected bipartite metabolite-reaction network was reconstructed using the KEGG database, comprising 8236 nodes (1601 metabolites and 6635 reactions). Simulated datasets with 75% sparsity mimicking two metabolic perturbations and two no-effect control groups, and real data from a previous study were used to test the strategy. Node-level statistical signal scores were distributed across the network topology using a random walk-based diffusion operator combined with a supervised clamping procedure to preserve initially observed measurements. A topological distance mask was subsequently applied to restrict propagation to nodes located within a predefined network distance from experimentally measured metabolites.
Results:
Network propagation redistributed statistical relevance across connected subgraphs, expanding the number of metabolic features carrying topology-informed statistical scores beyond experimentally observed metabolites in simulated and real data. In both oxidative stress and mitochondrial dysfunction simulations, significant nodes displayed non-random topological organization consistent with the simulated perturbations. Propagation of real data also identified additional unmeasured metabolites that were topologically connected to experimentally observed metabolites. In multivariate analyses, network propagation expanded the feature space and showed that it could improve clustering performance.
Conclusions:
The proposed bipartite metabolite-reaction network propagation provides an exploratory tool for metabolomics. By integrating topological context with statistical relevance, this approach complements established pathway analyses for hypothesis generation and candidate feature recovery in partially observed metabolic systems.
