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Published on: March 8, 2012
Analysis of EBV Infection and Gene Integration in Cervical Exfoliated Cells from HPV-Positive Women
Liang Lai1,2, Lu Ruijiao1, Yangchun Feng1,3
1Clinical Laboratory Center, Cancer Hospital Affiliated to Xinjiang Medical University, Ürümqi, Xijiang, People's Republic of China.
Purpose:
Epstein-Barr virus (EBV) infection and gene integration have been detected in cervical cancer tissues, but the association between the two has not yet been confirmed. This study employed a high-throughput viral integration detection method to analyze EBV infection and integration status in cervical exfoliated cells from 88 HPV-positive women, investigating whether a correlation exists between the two.
Patients And Methods:
Cervical exfoliated cell samples from 88 HPV-positive female patients were obtained from the Cancer Hospital Affiliated to Xinjiang Medical University. Liquid-based cytology was diagnosed by hospital pathologists, followed by high-throughput detection to evaluate EBV and HPV infection and integration.
Results:
We found that EBV was detected in 34.09% (30/88) of cervical exfoliated cells from HPV-positive females. The EBV positivity rate was 31.65% (25/79) in cases with multiple HPV infections and 55.56% (5/9) in those with single HPV subtype infection. The EBV infection rate in samples with HPV16/18 subtype infection was 41.86% (18/43), while in samples without HPV16/18 subtype infection, the EBV infection rate was 26.67% (12/45). According to the 2019 guidelines of the American Society for Colposcopy and Cervical Pathology (ASCCP), based on the risk of developing CIN III or higher lesions within five years, the EBV infection rates were 25.93% (7/27) in the normal group, 32.35% (11/34) in the low-risk group, and 44.44% (12/27) in the high-risk group. The most common sites for EBV self-integration occur within protein-coding regions, while integrations at repetitive sequences are relatively rare. In cases of HPV and EBV co-infection, HPV and EBV exhibit correlations in integration events and the distribution of integrated genes on host chromosomes. Under high-risk conditions, the number of EBV integrations, the genes involved in integration, and differentially expressed genes all exhibit significant differences.
Conclusion:
EBV potentially contributes to cervical cancer progression, and additional research and experimental verification are required.
