Related Experiment Video
Updated: Sep 19, 2026
Integrated Bone Formation Through In Vivo Endochondral Ossification Using Mesenchymal Stem Cells
Published on: July 14, 2023
Photo-crosslinked gelatin methacryloyl/methacrylated chitosan Janus membrane with covalently integrated bilayers for
Jingwen Wu1, Xueling Xiao2, Xinghai Wang3
1State Key Laboratory of Oral Diseases & National Center for Stomatology & National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, Sichuan, China.
Abstract:
Alveolar bone regeneration remains a significant clinical challenge, largely because conventional guided bone regeneration (GBR) membranes lack the ability to provide robust, integrated mechanical support and potent osteogenic bioactivity. To address these limitations, we designed a bilayer Janus membrane (G5CS1-G10). It comprises a dense, photo-crosslinked gelatin methacryloyl (GelMA) layer for mechanical support and a porous composite layer of GelMA with methacrylated chitosan (CSMA) for osteogenesis. In a rat calvarial defect model, the G5CS1-G10 membrane significantly outperformed the commercial membrane Bio-Gide®, achieving a 1.5-fold increase in bone volume (BV/TV) and improved osseointegration. Our study demonstrates that this dual functional membrane successfully integrates barrier capacity with osteogenic promotion, offering a promising strategy for advanced maxillofacial bone regeneration.
