Complete genome sequence data of Acidisoma sibiricum DSM 21000^T with PHA pathway and a putative aerobic anoxygenic...

Acidisoma sibiricum DSM 21000^T, the type strain of an obligately aerobic, acidophilic, and psychrotolerant bacterium, was isolated from a Sphagnum-dominated peat bog in the Bakchar region of western Siberia, Russia. Here we report the complete genome sequence data of this type strain. Genomic DNA was sequenced using both Illumina NovaSeq X (paired-end, 2 × 150 bp) and PacBio Revio platforms. Hybrid assembly yielded a complete genome consisting of one chromosome (3925,831 bp) and two plasmids, pAS1 (577,300 bp) and pAS2 (19,537 bp), with a total genome size of 4522,668 bp and a sequencing coverage of 312×. The genome was found to be 98.97% complete with an estimated contamination of 2.25%. This is the first report of a complete genome sequence for the type strain of A. sibiricum. Digital DNA-DNA hybridisation (dDDH) analysis confirmed that DSM 21,000 is genomically distinct from the closest described Acidisoma species tested, with the highest dDDH value of 25.0% with A. cellulosilyticum HW T5.17, well below the 70% species delineation threshold. Genome annotation by NCBI PGAP revealed 4225 genes, including 4127 protein-coding sequences. A complete set of polyhydroxyalkanoate (PHA) biosynthesis genes was also identified, consistent with the presence of intracellular poly-β-hydroxybutyrate granules previously reported for this species. Furthermore, a putative aerobic anoxygenic photosynthesis (AAP) gene cluster was identified on the chromosome, comprising genes encoding a type II photosynthetic reaction center, a light-harvesting antenna complex LH1, and a complete bacteriochlorophyll a biosynthesis pathway. All sequence data were deposited in the NCBI GenBank database under BioProject PRJNA1302119.