Related Experiment Video
Updated: Sep 19, 2026
Dissociation of Human and Mouse Tumor Tissue Samples for Single-cell RNA Sequencing
Published on: August 16, 2024
Single-cell RNA sequencing reveals compartment-specific immune and tumor cell states in a contiguous spinal
Yassmin A Elbanna1, Kenny K H Yu2, Tejus A Bale3
1Louis V. Gerstner, Jr., Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, New York, USA.
Background:
Metastatic disease commonly exhibits differential treatment responses across anatomical sites, including visceral versus osseous lesions and, within the spine, osseous, epidural, and intradural compartments. Intradural spinal metastases, though rare, portend rapid neurologic deterioration and worse outcomes than epidural disease, yet the biological mechanisms underlying this differential behavior remain poorly understood.
Methods:
We report a 61-year-old woman with metastatic ER+/PR+ breast cancer who developed bilateral leg weakness and loss of walking due to severe thoracic spinal cord compression. Imaging showed progressive epidural and intradural tumors, whereas osseous metastases remained stable. We performed single-cell RNA sequencing on tumor samples from both compartments after surgical decompression, analyzing cell types, immune subclustering, gene expression, and tumor module scores.
Results:
Sequencing revealed 12 distinct cell populations in both epidural and intradural metastases, including tumor epithelial, neural, glial, macrophages, myeloid, cytotoxic T, NK, and proliferating cells. While major cell types were similar, their composition and gene expression differed. Epidural cytotoxic T and NK cells expressed higher levels of GZMB, PRF1, NKG7, KLRD1, and GNLY. Macrophages split into 2 groups: a C1QA/C1QB-enriched subset mainly in the intradural compartment and an activated, inflammatory, antigen-presenting group enriched in the epidural compartment with IL1B, HLA-DRA, CD74, and other genes. Tumor epithelial cells also differed: intradural cells showed increased immune gene expression, antigen presentation, and interferon expression.
Conclusion:
The study shows cellular and transcriptional differences within a single spinal metastasis, influenced by local anatomy. These findings suggest that the microenvironment impacts immune and tumor states, guiding future research on metastasis and therapy responses.
